miRNA: 3'- gCGU-GCC-ACUGGaa-CGGUAGUUgCUu -5'
miRNA: 3'- gCGUGCCA------------CUGGAa---CG-GUAGUUGCUU- -5'
miRNA: 3'- -GCGUGCCACUGGAACGGUAGUUGCUU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18262 | 3' | -53.5 | NC_004681.1 | + | 60058 | 0.72 | 0.510334 |
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Target: 5'- gGCAcCGGcUGACCgugGCCAUCGAgGAc -3' miRNA: 3'- gCGU-GCC-ACUGGaa-CGGUAGUUgCUu -5' |
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| 18262 | 3' | -53.5 | NC_004681.1 | + | 44740 | 0.75 | 0.349049 |
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Target: 5'- uGCACGGUgcagucgguguaGACCUggugGCuCAUCAGCGAGc -3' miRNA: 3'- gCGUGCCA------------CUGGAa---CG-GUAGUUGCUU- -5' |
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| 18262 | 3' | -53.5 | NC_004681.1 | + | 9583 | 1.07 | 0.002896 |
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Target: 5'- gCGCACGGUGACCUUGCCAUCAACGAAg -3' miRNA: 3'- -GCGUGCCACUGGAACGGUAGUUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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