Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18263 | 3' | -54.9 | NC_004681.1 | + | 56362 | 0.66 | 0.787305 |
Target: 5'- cUCGUGgccguUGAGGGuGUCGaAGGUGu -3' miRNA: 3'- aAGCACauuu-ACUCCCcCGGC-UCCAC- -5' |
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18263 | 3' | -54.9 | NC_004681.1 | + | 32556 | 0.67 | 0.747054 |
Target: 5'- cUUCGacgcccUGaAGGGGGCCGAGGa- -3' miRNA: 3'- -AAGCacauuuAC-UCCCCCGGCUCCac -5' |
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18263 | 3' | -54.9 | NC_004681.1 | + | 9652 | 0.69 | 0.596317 |
Target: 5'- ----cGUucGUGAGGGGGCCG-GGUc -3' miRNA: 3'- aagcaCAuuUACUCCCCCGGCuCCAc -5' |
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18263 | 3' | -54.9 | NC_004681.1 | + | 28613 | 0.71 | 0.52141 |
Target: 5'- -cCGUGgaGAUGAccgcGGGGGugcCCGGGGUGa -3' miRNA: 3'- aaGCACauUUACU----CCCCC---GGCUCCAC- -5' |
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18263 | 3' | -54.9 | NC_004681.1 | + | 22516 | 0.71 | 0.480246 |
Target: 5'- --gGUGUAGugcgGAGGGuugccguccgcGGCCGGGGUGa -3' miRNA: 3'- aagCACAUUua--CUCCC-----------CCGGCUCCAC- -5' |
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18263 | 3' | -54.9 | NC_004681.1 | + | 9773 | 1.06 | 0.002123 |
Target: 5'- uUUCGUGUAAAUGAGGGGGCCGAGGUGa -3' miRNA: 3'- -AAGCACAUUUACUCCCCCGGCUCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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