Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18263 | 5' | -51.8 | NC_004681.1 | + | 39196 | 0.66 | 0.945382 |
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Target: 5'- gCCCUCGacgucgaGGACGUCAGcCUGgUcgACUGu -3' miRNA: 3'- -GGGAGUg------CUUGCAGUC-GAUgGa-UGAC- -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 65928 | 0.66 | 0.945382 |
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Target: 5'- uCCCUCACGGugG-CA-CUgACCU-CUGg -3' miRNA: 3'- -GGGAGUGCUugCaGUcGA-UGGAuGAC- -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 44760 | 0.67 | 0.918323 |
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Target: 5'- uCCCUCGCGGGCGgcuUCAacucGUgggACCU-CUGg -3' miRNA: 3'- -GGGAGUGCUUGC---AGU----CGa--UGGAuGAC- -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 10010 | 0.67 | 0.905586 |
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Target: 5'- aUCUUCGgGGaagGCGUCGGCcAgCUGCUGg -3' miRNA: 3'- -GGGAGUgCU---UGCAGUCGaUgGAUGAC- -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 32976 | 0.68 | 0.869112 |
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Target: 5'- aCCCguaccgCGCGAAgggUGUCAGCUACCc---- -3' miRNA: 3'- -GGGa-----GUGCUU---GCAGUCGAUGGaugac -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 40865 | 0.68 | 0.852774 |
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Target: 5'- gUCCUCGCGGuacaAgGUgGGCUuaACCUGCUu -3' miRNA: 3'- -GGGAGUGCU----UgCAgUCGA--UGGAUGAc -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 10616 | 0.7 | 0.768962 |
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Target: 5'- aCgUCACGGACGUCuccCUGCCUgaugccACUGa -3' miRNA: 3'- gGgAGUGCUUGCAGuc-GAUGGA------UGAC- -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 14531 | 0.75 | 0.484837 |
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Target: 5'- gCCUCGCGGGCGgcguagauuuucgccUCGGCcGCCUGCUu -3' miRNA: 3'- gGGAGUGCUUGC---------------AGUCGaUGGAUGAc -5' |
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| 18263 | 5' | -51.8 | NC_004681.1 | + | 9739 | 1.12 | 0.002346 |
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Target: 5'- cCCCUCACGAACGUCAGCUACCUACUGg -3' miRNA: 3'- -GGGAGUGCUUGCAGUCGAUGGAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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