Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18264 | 3' | -53.8 | NC_004681.1 | + | 43787 | 0.66 | 0.855505 |
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Target: 5'- ---cGGCggCGGGGUGU-GUUGGAGGg -3' miRNA: 3'- cuaaCUGuaGUCCCGCGuCAGCCUCU- -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 39172 | 0.66 | 0.855505 |
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Target: 5'- ---gGGCuugCAcGGCGCGGUCGGAc- -3' miRNA: 3'- cuaaCUGua-GUcCCGCGUCAGCCUcu -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 22466 | 0.66 | 0.854672 |
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Target: 5'- ---cGACGUCGGGGagguccuCGCcGUgGGGGAu -3' miRNA: 3'- cuaaCUGUAGUCCC-------GCGuCAgCCUCU- -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 57455 | 0.67 | 0.811209 |
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Target: 5'- --cUGGCGUUAGcGCGCGGUCccuGGAGc -3' miRNA: 3'- cuaACUGUAGUCcCGCGUCAG---CCUCu -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 46950 | 0.68 | 0.772378 |
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Target: 5'- cGGUUGAuCAUCGGGGUGC---CGGGGc -3' miRNA: 3'- -CUAACU-GUAGUCCCGCGucaGCCUCu -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 60901 | 0.68 | 0.762285 |
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Target: 5'- cGUUGACGUagcuGGGGCGCuccuuGGUgGGAGc -3' miRNA: 3'- cUAACUGUAg---UCCCGCG-----UCAgCCUCu -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 24840 | 0.68 | 0.720706 |
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Target: 5'- ---cGGCGUCGGcGGCGCGGgCGGcGGc -3' miRNA: 3'- cuaaCUGUAGUC-CCGCGUCaGCCuCU- -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 22532 | 0.72 | 0.504108 |
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Target: 5'- ---gGACGuagaacuUCAGGGUGUAGUgCGGAGGg -3' miRNA: 3'- cuaaCUGU-------AGUCCCGCGUCA-GCCUCU- -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 63270 | 0.73 | 0.454607 |
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Target: 5'- ---gGACAUCAGGGUGUcgAG-CGGGGGa -3' miRNA: 3'- cuaaCUGUAGUCCCGCG--UCaGCCUCU- -5' |
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| 18264 | 3' | -53.8 | NC_004681.1 | + | 11418 | 1.08 | 0.002124 |
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Target: 5'- uGAUUGACAUCAGGGCGCAGUCGGAGAc -3' miRNA: 3'- -CUAACUGUAGUCCCGCGUCAGCCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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