Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18266 | 3' | -57.9 | NC_004681.1 | + | 69241 | 0.66 | 0.701315 |
Target: 5'- uGGUGUagccgAGCCGGUaggcgagggaggccaGGGCGCgGGCGa-- -3' miRNA: 3'- gCCACA-----UCGGCCA---------------CUCGCG-CUGCagu -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 25423 | 0.66 | 0.676393 |
Target: 5'- uCGGgaggGGUCGGUG-GCGUGGUGUCGa -3' miRNA: 3'- -GCCaca-UCGGCCACuCGCGCUGCAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 7498 | 0.67 | 0.644945 |
Target: 5'- cCGGUcgaugccugGGCCugGGUGcGCGCGACGUUc -3' miRNA: 3'- -GCCAca-------UCGG--CCACuCGCGCUGCAGu -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 4958 | 0.67 | 0.634428 |
Target: 5'- aGGcguugGUAGCCaucacugcGGUGGGUaCGGCGUCGa -3' miRNA: 3'- gCCa----CAUCGG--------CCACUCGcGCUGCAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 10298 | 0.67 | 0.634428 |
Target: 5'- aGGUGUGGCCGGUgcuccacucgGAGC-CGGggaggccggucgUGUCGa -3' miRNA: 3'- gCCACAUCGGCCA----------CUCGcGCU------------GCAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 54743 | 0.67 | 0.634428 |
Target: 5'- gGGUGUAGUCguagGGUGAGCG-GcCGUUc -3' miRNA: 3'- gCCACAUCGG----CCACUCGCgCuGCAGu -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 10274 | 0.67 | 0.62391 |
Target: 5'- cCGGU---GCCGGaUGAGCugauCGACGUCGc -3' miRNA: 3'- -GCCAcauCGGCC-ACUCGc---GCUGCAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 32024 | 0.67 | 0.596615 |
Target: 5'- gGGUGUcggcacGGCCGGUGugauccugggcaagcAGCGCaaggaaGGCGUCc -3' miRNA: 3'- gCCACA------UCGGCCAC---------------UCGCG------CUGCAGu -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 12413 | 0.68 | 0.581986 |
Target: 5'- uCGGUG-AGCCGGUGAcggacgaggacgGCGaaguGACGaUCAc -3' miRNA: 3'- -GCCACaUCGGCCACU------------CGCg---CUGC-AGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 12996 | 0.69 | 0.520366 |
Target: 5'- uCGGcacGaAGCCGGUGuGgGUGugGUCAc -3' miRNA: 3'- -GCCa--CaUCGGCCACuCgCGCugCAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 14492 | 0.69 | 0.510329 |
Target: 5'- aGGgcgcgcugGUGGCCGGUGcuGGCGCGAgcaaggugaaGUCGa -3' miRNA: 3'- gCCa-------CAUCGGCCAC--UCGCGCUg---------CAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 5636 | 0.69 | 0.490505 |
Target: 5'- cCGGUGUgggcacgcAGCUGGUGAGCuucgcCGACGaCGa -3' miRNA: 3'- -GCCACA--------UCGGCCACUCGc----GCUGCaGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 67474 | 0.7 | 0.461465 |
Target: 5'- aGGgg-AGCgCGGUGAGCGgGACGa-- -3' miRNA: 3'- gCCacaUCG-GCCACUCGCgCUGCagu -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 48521 | 0.7 | 0.446352 |
Target: 5'- gGGUGgcGCacugccaauacuucaCGGUcGAGCGCGACGgCAg -3' miRNA: 3'- gCCACauCG---------------GCCA-CUCGCGCUGCaGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 51306 | 0.7 | 0.415182 |
Target: 5'- ----aUGGCCGGUGAGUccGcCGACGUCAu -3' miRNA: 3'- gccacAUCGGCCACUCG--C-GCUGCAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 18210 | 0.72 | 0.363571 |
Target: 5'- cCGGUGccUGGCCGG-GGGCGcCGAUGcCGg -3' miRNA: 3'- -GCCAC--AUCGGCCaCUCGC-GCUGCaGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 54388 | 0.72 | 0.363571 |
Target: 5'- gGGUGUGGaguucgaGGUGgucgacgucaAGCGCGACGUCc -3' miRNA: 3'- gCCACAUCgg-----CCAC----------UCGCGCUGCAGu -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 17926 | 0.73 | 0.316579 |
Target: 5'- uGGUGaGGCCGGUGccuuGCGCGGCcgccgcguugauGUCGg -3' miRNA: 3'- gCCACaUCGGCCACu---CGCGCUG------------CAGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 27324 | 0.73 | 0.309206 |
Target: 5'- uGGUGUucacGGCCaGGagGGGCGCGACGcCGg -3' miRNA: 3'- gCCACA----UCGG-CCa-CUCGCGCUGCaGU- -5' |
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18266 | 3' | -57.9 | NC_004681.1 | + | 13077 | 0.97 | 0.006359 |
Target: 5'- gCGGUGUAGCCGGUGAGCGC-ACGUCAg -3' miRNA: 3'- -GCCACAUCGGCCACUCGCGcUGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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