Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18266 | 5' | -55.6 | NC_004681.1 | + | 67329 | 0.66 | 0.794018 |
Target: 5'- --cGACcugGCCGUgucgCAGGGCCUg-- -3' miRNA: 3'- gcaCUGcaaCGGCGa---GUCCCGGAaag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 13683 | 0.66 | 0.794018 |
Target: 5'- -uUGGCGUgGCCGCcCGcGGCCUUg- -3' miRNA: 3'- gcACUGCAaCGGCGaGUcCCGGAAag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 36872 | 0.66 | 0.788258 |
Target: 5'- --cGGCGUUGaucaggucuauugcaUCGCUCAGGGUCa--- -3' miRNA: 3'- gcaCUGCAAC---------------GGCGAGUCCCGGaaag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 33321 | 0.66 | 0.754624 |
Target: 5'- cCGgGACGUUcGCCGUcCAGGGCa---- -3' miRNA: 3'- -GCaCUGCAA-CGGCGaGUCCCGgaaag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 65216 | 0.66 | 0.754624 |
Target: 5'- uGUGGCG--GCCGUacuUCAGGGCgCcUUCc -3' miRNA: 3'- gCACUGCaaCGGCG---AGUCCCG-GaAAG- -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 52305 | 0.67 | 0.734162 |
Target: 5'- -aUGGCGggGCCGUUCuuGGCCguggaUUUCg -3' miRNA: 3'- gcACUGCaaCGGCGAGucCCGG-----AAAG- -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 54412 | 0.67 | 0.727943 |
Target: 5'- gGUGGCGUcgaugacgacggaugUGCCGCUCuugagguGGGCg---- -3' miRNA: 3'- gCACUGCA---------------ACGGCGAGu------CCCGgaaag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 51235 | 0.68 | 0.627644 |
Target: 5'- --gGugGUUGCUcauucaCUCAGGGCCUUc- -3' miRNA: 3'- gcaCugCAACGGc-----GAGUCCCGGAAag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 67354 | 0.69 | 0.584586 |
Target: 5'- --cGGCGUUGCgGUgcggCGGGGCCa--- -3' miRNA: 3'- gcaCUGCAACGgCGa---GUCCCGGaaag -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 51510 | 0.69 | 0.563253 |
Target: 5'- uCGUGGCGUUGagCGCggAGGGCacgUUCu -3' miRNA: 3'- -GCACUGCAACg-GCGagUCCCGga-AAG- -5' |
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18266 | 5' | -55.6 | NC_004681.1 | + | 13041 | 1.09 | 0.001378 |
Target: 5'- gCGUGACGUUGCCGCUCAGGGCCUUUCc -3' miRNA: 3'- -GCACUGCAACGGCGAGUCCCGGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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