miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18267 5' -54.8 NC_004681.1 + 69516 0.66 0.82646
Target:  5'- cCACGCCGUgug-CUcacUGcUGCGGCggCCGu -3'
miRNA:   3'- -GUGCGGCAacuaGA---AC-ACGCCGa-GGU- -5'
18267 5' -54.8 NC_004681.1 + 17594 0.66 0.789059
Target:  5'- aGCGCCGUgguAUCg---GCGGCUCgGg -3'
miRNA:   3'- gUGCGGCAac-UAGaacaCGCCGAGgU- -5'
18267 5' -54.8 NC_004681.1 + 53048 0.67 0.759302
Target:  5'- -cCGCCGa-GGUCcucGUGCGGCUCUu -3'
miRNA:   3'- guGCGGCaaCUAGaa-CACGCCGAGGu -5'
18267 5' -54.8 NC_004681.1 + 43302 0.67 0.749115
Target:  5'- uCGCGCUGUUGuuuuGUCUgagcGUGUGGCauaaggCCAg -3'
miRNA:   3'- -GUGCGGCAAC----UAGAa---CACGCCGa-----GGU- -5'
18267 5' -54.8 NC_004681.1 + 62165 0.69 0.605756
Target:  5'- cCAUGCCGcgcacagucaucgUGGUCUUGUGCGuGC-CCu -3'
miRNA:   3'- -GUGCGGCa------------ACUAGAACACGC-CGaGGu -5'
18267 5' -54.8 NC_004681.1 + 19779 0.7 0.577674
Target:  5'- gACGCUGg------UGUGCGGCUCCGc -3'
miRNA:   3'- gUGCGGCaacuagaACACGCCGAGGU- -5'
18267 5' -54.8 NC_004681.1 + 13595 1.08 0.001795
Target:  5'- cCACGCCGUUGAUCUUGUGCGGCUCCAc -3'
miRNA:   3'- -GUGCGGCAACUAGAACACGCCGAGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.