Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18269 | 5' | -56.3 | NC_004681.1 | + | 27798 | 0.71 | 0.438611 |
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Target: 5'- -aCUUCUGgAGAuGGGCGcGGCGgCGCc -3' miRNA: 3'- ugGAAGACgUCUuCCCGC-CUGCaGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 21396 | 0.71 | 0.428322 |
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Target: 5'- cGCCUUCUucaGCGGGAacaggaggcccuuGGGCuGGGgGUCGCc -3' miRNA: 3'- -UGGAAGA---CGUCUU-------------CCCG-CCUgCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 40743 | 0.72 | 0.41089 |
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Target: 5'- cGCCUUaCgugGCGGAguagcugggagAGGGCGGccaccaucaACGUCGCc -3' miRNA: 3'- -UGGAA-Ga--CGUCU-----------UCCCGCC---------UGCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 52381 | 0.72 | 0.384284 |
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Target: 5'- gGCCUUCUGgAGGAGGa-GGacaacguccGCGUCGCg -3' miRNA: 3'- -UGGAAGACgUCUUCCcgCC---------UGCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 49962 | 0.74 | 0.319125 |
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Target: 5'- gGCgUUUCUGCGGGguucugccaGGGGCGGACGUgaaaGCc -3' miRNA: 3'- -UG-GAAGACGUCU---------UCCCGCCUGCAg---CG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 14144 | 0.74 | 0.319125 |
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Target: 5'- cCCUUCUGCAGAAGGuGCaGGGCaucuUCGg -3' miRNA: 3'- uGGAAGACGUCUUCC-CG-CCUGc---AGCg -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 20376 | 0.74 | 0.296922 |
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Target: 5'- -aCUgCUGCAGGAGcGGCGGgACGUUGUu -3' miRNA: 3'- ugGAaGACGUCUUC-CCGCC-UGCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 14235 | 1.11 | 0.000885 |
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Target: 5'- cACCUUCUGCAGAAGGGCGGACGUCGCu -3' miRNA: 3'- -UGGAAGACGUCUUCCCGCCUGCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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