Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18274 | 3' | -55.7 | NC_004681.1 | + | 28752 | 0.72 | 0.434985 |
Target: 5'- uUGAGACCgACCGUGGGUCcgauaguUCcGCCu -3' miRNA: 3'- gGCUCUGG-UGGUACCCAGu------AGuCGGu -5' |
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18274 | 3' | -55.7 | NC_004681.1 | + | 15914 | 0.72 | 0.416451 |
Target: 5'- aCCGAG-CCACUGUGcgacguugcggaGGcCGUCAGCCAu -3' miRNA: 3'- -GGCUCuGGUGGUAC------------CCaGUAGUCGGU- -5' |
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18274 | 3' | -55.7 | NC_004681.1 | + | 8521 | 0.75 | 0.316051 |
Target: 5'- gUGAGACCGCCcccGcGGcCAUCGGCCAg -3' miRNA: 3'- gGCUCUGGUGGua-C-CCaGUAGUCGGU- -5' |
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18274 | 3' | -55.7 | NC_004681.1 | + | 16371 | 0.75 | 0.301203 |
Target: 5'- aCGAGGgCGCCAagUGGGUUGccUCGGCCAc -3' miRNA: 3'- gGCUCUgGUGGU--ACCCAGU--AGUCGGU- -5' |
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18274 | 3' | -55.7 | NC_004681.1 | + | 22554 | 0.78 | 0.205695 |
Target: 5'- aCGAGGCCACCcagaagauggucUGGGUCGcCAGCCGc -3' miRNA: 3'- gGCUCUGGUGGu-----------ACCCAGUaGUCGGU- -5' |
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18274 | 3' | -55.7 | NC_004681.1 | + | 62571 | 0.8 | 0.142835 |
Target: 5'- uUCGuAGACCACCGUgGGGUCGugccccUCGGCCAu -3' miRNA: 3'- -GGC-UCUGGUGGUA-CCCAGU------AGUCGGU- -5' |
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18274 | 3' | -55.7 | NC_004681.1 | + | 16534 | 1.1 | 0.001127 |
Target: 5'- gCCGAGACCACCAUGGGUCAUCAGCCAg -3' miRNA: 3'- -GGCUCUGGUGGUACCCAGUAGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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