Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18276 | 5' | -55.7 | NC_004681.1 | + | 69629 | 0.69 | 0.564747 |
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Target: 5'- uCgGGGCAcGGCCGCCGcAGCAGuGAg-- -3' miRNA: 3'- uGgUUCGU-CCGGCGGC-UUGUC-CUaaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 33113 | 0.69 | 0.564747 |
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Target: 5'- uGCCAugucccgaggucGGCAGGCCGCUac-CGGGGUa- -3' miRNA: 3'- -UGGU------------UCGUCCGGCGGcuuGUCCUAaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 60136 | 0.69 | 0.553988 |
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Target: 5'- uGCCAGGUgcgGGaGCCGCCGAaggcgaGCAGGu--- -3' miRNA: 3'- -UGGUUCG---UC-CGGCGGCU------UGUCCuaaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 11283 | 0.69 | 0.553988 |
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Target: 5'- gUCAAG-AGGCUGCCGggUGGGGg-- -3' miRNA: 3'- uGGUUCgUCCGGCGGCuuGUCCUaaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 29583 | 0.69 | 0.532661 |
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Target: 5'- cACCGAGguGGUC-CCGGugAGGGa-- -3' miRNA: 3'- -UGGUUCguCCGGcGGCUugUCCUaaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 9082 | 0.69 | 0.532661 |
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Target: 5'- gGCCGAGguGG-CGCCGAcaGCgAGGAg-- -3' miRNA: 3'- -UGGUUCguCCgGCGGCU--UG-UCCUaaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 9039 | 0.7 | 0.501253 |
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Target: 5'- aGCCGgucgaacgcugcAGCAGGCCGuuGAcgcCGGGGUa- -3' miRNA: 3'- -UGGU------------UCGUCCGGCggCUu--GUCCUAaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 62097 | 0.71 | 0.421966 |
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Target: 5'- gUCGAGUAGGCCGCgaucugguCGAACAGGu--- -3' miRNA: 3'- uGGUUCGUCCGGCG--------GCUUGUCCuaaa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 20483 | 0.72 | 0.359136 |
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Target: 5'- cUCAGGCGGGCCGucuuCCGGcuucgGCGGGAUUg -3' miRNA: 3'- uGGUUCGUCCGGC----GGCU-----UGUCCUAAa -5' |
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| 18276 | 5' | -55.7 | NC_004681.1 | + | 17236 | 1.05 | 0.002146 |
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Target: 5'- gACCAAGCAGGCCGCCGAACAGGAUUUc -3' miRNA: 3'- -UGGUUCGUCCGGCGGCUUGUCCUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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