Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18277 | 3' | -55 | NC_004681.1 | + | 45793 | 0.65 | 0.838013 |
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Target: 5'- uGGUAcUCGGCGAGGCggggCUUGAGUUc-- -3' miRNA: 3'- -UCGUcAGCCGCUCUG----GAACUCAGucc -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 17776 | 0.66 | 0.820366 |
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Target: 5'- cGUAGUCGG-GAGcuGCCggUGAGagCGGGa -3' miRNA: 3'- uCGUCAGCCgCUC--UGGa-ACUCa-GUCC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 3638 | 0.66 | 0.801962 |
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Target: 5'- gAGgAGuUCGGUGuuGugCgucgGAGUCAGGg -3' miRNA: 3'- -UCgUC-AGCCGCu-CugGaa--CUCAGUCC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 67534 | 0.67 | 0.77311 |
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Target: 5'- uGgAGUCGGUGAuguccggguuGGCCUUGAGcCAcuGGa -3' miRNA: 3'- uCgUCAGCCGCU----------CUGGAACUCaGU--CC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 19112 | 0.67 | 0.753162 |
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Target: 5'- cAGguGUCgccgucuuGGCGAGcacCCUUGcuuGUCAGGu -3' miRNA: 3'- -UCguCAG--------CCGCUCu--GGAACu--CAGUCC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 34273 | 0.68 | 0.690834 |
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Target: 5'- uGGgAGUCucaGGCGGGGCCUUcGGcuuuGUCGGGc -3' miRNA: 3'- -UCgUCAG---CCGCUCUGGAA-CU----CAGUCC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 44733 | 0.74 | 0.381044 |
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Target: 5'- uGCAGUCGGUGuAGACCUgguggcucaucagcGAG-CAGGa -3' miRNA: 3'- uCGUCAGCCGC-UCUGGAa-------------CUCaGUCC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 12507 | 0.77 | 0.244286 |
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Target: 5'- aGGCAGcaaCGGCGAGACCgccGAGUCgcAGGc -3' miRNA: 3'- -UCGUCa--GCCGCUCUGGaa-CUCAG--UCC- -5' |
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| 18277 | 3' | -55 | NC_004681.1 | + | 17453 | 1.1 | 0.001366 |
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Target: 5'- gAGCAGUCGGCGAGACCUUGAGUCAGGu -3' miRNA: 3'- -UCGUCAGCCGCUCUGGAACUCAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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