Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18278 | 5' | -54.2 | NC_004681.1 | + | 39180 | 0.72 | 0.534808 |
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Target: 5'- gGCCACCaGGGCUUgCAcGGCgCGGUcGGa -3' miRNA: 3'- -CGGUGG-CUUGAA-GUaCCGgGCCAaCC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 1643 | 0.72 | 0.514166 |
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Target: 5'- cGCCGCCcAGCUgaaggC-UGGCCCGGUcaccagGGg -3' miRNA: 3'- -CGGUGGcUUGAa----GuACCGGGCCAa-----CC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 16512 | 0.72 | 0.514166 |
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Target: 5'- aGCCAgCCGuAGCg-CAUGGCCUGGUcacccuUGGa -3' miRNA: 3'- -CGGU-GGC-UUGaaGUACCGGGCCA------ACC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 29586 | 0.75 | 0.38973 |
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Target: 5'- aGCCACCGAG----GUGGuCCCGGUgagGGa -3' miRNA: 3'- -CGGUGGCUUgaagUACC-GGGCCAa--CC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 13134 | 0.77 | 0.2867 |
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Target: 5'- cGCCACCGccCUUCGUGGCaCGGcgGGu -3' miRNA: 3'- -CGGUGGCuuGAAGUACCGgGCCaaCC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 18564 | 1.12 | 0.001435 |
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Target: 5'- gGCCACCGAACUUCAUGGCCCGGUUGGc -3' miRNA: 3'- -CGGUGGCUUGAAGUACCGGGCCAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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