Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18281 | 3' | -57.3 | NC_004681.1 | + | 68585 | 0.66 | 0.678315 |
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Target: 5'- aGCgacagCGGGCCAUaguccUCGUaggAGCGGAGc -3' miRNA: 3'- aUGa----GCCCGGUGaa---AGCG---UCGCCUCa -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 1541 | 0.66 | 0.64614 |
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Target: 5'- gAC-CGGGCCAgCcUUCagcuggGCGGCGGGGg -3' miRNA: 3'- aUGaGCCCGGU-GaAAG------CGUCGCCUCa -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 34535 | 0.66 | 0.64614 |
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Target: 5'- aACUCGGGCgccgcgguggcgCGCUucUUCGguGUGGAc- -3' miRNA: 3'- aUGAGCCCG------------GUGA--AAGCguCGCCUca -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 30148 | 0.66 | 0.635377 |
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Target: 5'- gGgUCGGGCUcagGCgg-CGCGgccGCGGAGUg -3' miRNA: 3'- aUgAGCCCGG---UGaaaGCGU---CGCCUCA- -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 16461 | 0.67 | 0.571054 |
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Target: 5'- cACUUGGcgcccucgucGCCACccaccgcCGCAGCGGAGa -3' miRNA: 3'- aUGAGCC----------CGGUGaaa----GCGUCGCCUCa -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 30340 | 0.68 | 0.539426 |
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Target: 5'- gGCUCGGGg-GCUUUCGCAuUGGGGg -3' miRNA: 3'- aUGAGCCCggUGAAAGCGUcGCCUCa -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 67358 | 0.68 | 0.518673 |
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Target: 5'- aACUCGGcGUUGCgg-UGCGGCGGGGc -3' miRNA: 3'- aUGAGCC-CGGUGaaaGCGUCGCCUCa -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 70939 | 0.71 | 0.375878 |
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Target: 5'- aUGCUCGcGGCCGCUga-GC-GUGGAGa -3' miRNA: 3'- -AUGAGC-CCGGUGAaagCGuCGCCUCa -5' |
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| 18281 | 3' | -57.3 | NC_004681.1 | + | 20573 | 1.05 | 0.001612 |
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Target: 5'- cUACUCGGGCCACUUUCGCAGCGGAGUu -3' miRNA: 3'- -AUGAGCCCGGUGAAAGCGUCGCCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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