Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18282 | 3' | -54.9 | NC_004681.1 | + | 48689 | 0.66 | 0.81279 |
Target: 5'- cGGAUGC-CUgccUCUGCGAGggcguggcCCACGAUGc -3' miRNA: 3'- -CCUGCGaGA---GGACGUUCa-------GGUGCUGC- -5' |
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18282 | 3' | -54.9 | NC_004681.1 | + | 74819 | 0.66 | 0.816427 |
Target: 5'- cGGAUGCUCcagugCUUGCAagcguugcucuaugaGGUCUACaGCGg -3' miRNA: 3'- -CCUGCGAGa----GGACGU---------------UCAGGUGcUGC- -5' |
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18282 | 3' | -54.9 | NC_004681.1 | + | 50059 | 0.66 | 0.821827 |
Target: 5'- cGACGCcaUCgacugCCUGCGGGUCgACGuCc -3' miRNA: 3'- cCUGCG--AGa----GGACGUUCAGgUGCuGc -5' |
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18282 | 3' | -54.9 | NC_004681.1 | + | 6629 | 0.66 | 0.839325 |
Target: 5'- gGGugGCU--UCUGCAugAGgcgCCGCGGCu -3' miRNA: 3'- -CCugCGAgaGGACGU--UCa--GGUGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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