Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18282 | 3' | -54.9 | NC_004681.1 | + | 30906 | 0.68 | 0.724585 |
Target: 5'- cGGACG-UCUCCgaaugGCAAGUgCCGguCGAUGu -3' miRNA: 3'- -CCUGCgAGAGGa----CGUUCA-GGU--GCUGC- -5' |
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18282 | 3' | -54.9 | NC_004681.1 | + | 7239 | 0.67 | 0.803575 |
Target: 5'- -cGCGCuUCUCCagGCca-UCCACGACGc -3' miRNA: 3'- ccUGCG-AGAGGa-CGuucAGGUGCUGC- -5' |
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18282 | 3' | -54.9 | NC_004681.1 | + | 50059 | 0.66 | 0.821827 |
Target: 5'- cGACGCcaUCgacugCCUGCGGGUCgACGuCc -3' miRNA: 3'- cCUGCG--AGa----GGACGUUCAGgUGCuGc -5' |
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18282 | 3' | -54.9 | NC_004681.1 | + | 3061 | 0.73 | 0.44443 |
Target: 5'- uGGugGCagUCCUGCGcaagaugggAGUCCuCGGCGc -3' miRNA: 3'- -CCugCGagAGGACGU---------UCAGGuGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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