miRNA: 3'- -ACUCGUGCUCUca--CUGUUGUGGGcag -5'
miRNA: 3'- -ACUCGUGCUCuCACU-GUUGUGGGCaG- -5'
miRNA: 3'- aCUCg---UgCUCUCaCUG-UUGUG-GGCAG- -5'
miRNA: 3'- aCUCGUGCUCuCACUGU--UGUGGGCag -5'
miRNA: 3'- -ACUCGUGCU--------CUCaCUGU---------UGUGGGcAG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18283 | 5' | -53.6 | NC_004681.1 | + | 50358 | 0.72 | 0.529958 |
|
Target: 5'- gUGGGCGCGGGAccagGACAGCGCCUu-- -3' miRNA: 3'- -ACUCGUGCUCUca--CUGUUGUGGGcag -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 60184 | 0.84 | 0.106636 |
|
Target: 5'- cUGAGCGCGAG-GUGAuCGGCACCCGcCg -3' miRNA: 3'- -ACUCGUGCUCuCACU-GUUGUGGGCaG- -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 61305 | 0.66 | 0.881809 |
|
Target: 5'- cGAGaccaAgGAGGGcGACaAGCGCaCCGUCa -3' miRNA: 3'- aCUCg---UgCUCUCaCUG-UUGUG-GGCAG- -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 64931 | 0.78 | 0.24665 |
|
Target: 5'- gGAGCACGAG-GUGGCGacGCGCCUGg- -3' miRNA: 3'- aCUCGUGCUCuCACUGU--UGUGGGCag -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 68178 | 0.66 | 0.858204 |
|
Target: 5'- aUGAGCGCGAauucccaaGAGgGGCAccacuucuuGCGCCCaUCg -3' miRNA: 3'- -ACUCGUGCU--------CUCaCUGU---------UGUGGGcAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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