miRNA: 3'- -ACUCGU----GCUCUCaCuGUUGUGGGCAG- -5'
miRNA: 3'- aCUCGUGCUCUCACUGuuGUGGGCaG- -5'
miRNA: 3'- aCUCGUGCUCuCACUGU--UGUGGGCag -5'
miRNA: 3'- -ACUCGUGCUCuCACU-GUUGUGGGCaG- -5'
miRNA: 3'- -ACUCGUGCUCUCACUGUUGUGGGCAG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18283 | 5' | -53.6 | NC_004681.1 | + | 23280 | 0.75 | 0.374574 |
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Target: 5'- uUGAGCAacccUGAGAG-GuCGAUGCCCGUCg -3' miRNA: 3'- -ACUCGU----GCUCUCaCuGUUGUGGGCAG- -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 23598 | 0.75 | 0.365923 |
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Target: 5'- aGGGCACGGGGGUGGC--CAUCCGcCu -3' miRNA: 3'- aCUCGUGCUCUCACUGuuGUGGGCaG- -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 64931 | 0.78 | 0.24665 |
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Target: 5'- gGAGCACGAG-GUGGCGacGCGCCUGg- -3' miRNA: 3'- aCUCGUGCUCuCACUGU--UGUGGGCag -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 60184 | 0.84 | 0.106636 |
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Target: 5'- cUGAGCGCGAG-GUGAuCGGCACCCGcCg -3' miRNA: 3'- -ACUCGUGCUCuCACU-GUUGUGGGCaG- -5' |
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| 18283 | 5' | -53.6 | NC_004681.1 | + | 22347 | 1.1 | 0.001867 |
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Target: 5'- gUGAGCACGAGAGUGACAACACCCGUCu -3' miRNA: 3'- -ACUCGUGCUCUCACUGUUGUGGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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