Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18288 | 3' | -54.3 | NC_004681.1 | + | 13130 | 0.66 | 0.83096 |
Target: 5'- uGCCCGcCACCGCccuucgUGgcacgGCGGGUaCGGg- -3' miRNA: 3'- -CGGGU-GUGGUGa-----AUa----CGUCCA-GCCag -5' |
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18288 | 3' | -54.3 | NC_004681.1 | + | 7004 | 0.67 | 0.783145 |
Target: 5'- aGCCCACgguggugGCCACggacAUGCcguGGUCGacGUCg -3' miRNA: 3'- -CGGGUG-------UGGUGaa--UACGu--CCAGC--CAG- -5' |
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18288 | 3' | -54.3 | NC_004681.1 | + | 3135 | 0.66 | 0.848328 |
Target: 5'- gGCCCuugGCGCCGCggGUGCGGG-Ca--- -3' miRNA: 3'- -CGGG---UGUGGUGaaUACGUCCaGccag -5' |
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18288 | 3' | -54.3 | NC_004681.1 | + | 423 | 0.68 | 0.742785 |
Target: 5'- aGCCCucgcagaaauccgGCAgCCcguGCUUccGCGGGUCGGUg -3' miRNA: 3'- -CGGG-------------UGU-GG---UGAAuaCGUCCAGCCAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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