Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18288 | 5' | -59.8 | NC_004681.1 | + | 67368 | 0.66 | 0.554709 |
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Target: 5'- cACGA-CGgagaACUCGGCGUugcGGUGCGGCgGg -3' miRNA: 3'- -UGUUaGC----UGGGCCGCG---UCACGCCGgU- -5' |
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| 18288 | 5' | -59.8 | NC_004681.1 | + | 40570 | 0.66 | 0.575385 |
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Target: 5'- gGCGgcGUCGGCCgCGGCGUucaauGUcauCGGCCGc -3' miRNA: 3'- -UGU--UAGCUGG-GCCGCGu----CAc--GCCGGU- -5' |
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| 18288 | 5' | -59.8 | NC_004681.1 | + | 28031 | 0.66 | 0.575385 |
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Target: 5'- aGCAAgaccgaCGGCgCUGGCGCuG-GCGGUCGa -3' miRNA: 3'- -UGUUa-----GCUG-GGCCGCGuCaCGCCGGU- -5' |
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| 18288 | 5' | -59.8 | NC_004681.1 | + | 3399 | 0.66 | 0.575385 |
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Target: 5'- -aAGUUGGCCgCGGCGCccgcGCGaGCCAc -3' miRNA: 3'- ugUUAGCUGG-GCCGCGuca-CGC-CGGU- -5' |
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| 18288 | 5' | -59.8 | NC_004681.1 | + | 61355 | 0.66 | 0.575385 |
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Target: 5'- -aGAUCGGCCCGucGCuGCGGUG-GGCa- -3' miRNA: 3'- ugUUAGCUGGGC--CG-CGUCACgCCGgu -5' |
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| 18288 | 5' | -59.8 | NC_004681.1 | + | 11093 | 0.66 | 0.575385 |
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Target: 5'- cGCgAAUCGACuuGGCGUAGU-CGcCCGg -3' miRNA: 3'- -UG-UUAGCUGggCCGCGUCAcGCcGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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