Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18288 | 5' | -59.8 | NC_004681.1 | + | 11093 | 0.66 | 0.575385 |
Target: 5'- cGCgAAUCGACuuGGCGUAGU-CGcCCGg -3' miRNA: 3'- -UG-UUAGCUGggCCGCGUCAcGCcGGU- -5' |
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18288 | 5' | -59.8 | NC_004681.1 | + | 8468 | 0.67 | 0.514078 |
Target: 5'- gGCGGcUGAcCCCGGCaCGGcUGCGGCUg -3' miRNA: 3'- -UGUUaGCU-GGGCCGcGUC-ACGCCGGu -5' |
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18288 | 5' | -59.8 | NC_004681.1 | + | 7375 | 0.68 | 0.455603 |
Target: 5'- cGCAGgcggCGcACUCGGCGCcgaGGUGgaCGGCCGc -3' miRNA: 3'- -UGUUa---GC-UGGGCCGCG---UCAC--GCCGGU- -5' |
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18288 | 5' | -59.8 | NC_004681.1 | + | 4188 | 0.69 | 0.358645 |
Target: 5'- cACGAgaggaagCGACCCaGCGCGggguuggcGUGgGGCCAg -3' miRNA: 3'- -UGUUa------GCUGGGcCGCGU--------CACgCCGGU- -5' |
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18288 | 5' | -59.8 | NC_004681.1 | + | 3507 | 0.69 | 0.392202 |
Target: 5'- cGCGcgUGGCUC-GCGCGGgcgccGCGGCCAa -3' miRNA: 3'- -UGUuaGCUGGGcCGCGUCa----CGCCGGU- -5' |
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18288 | 5' | -59.8 | NC_004681.1 | + | 3399 | 0.66 | 0.575385 |
Target: 5'- -aAGUUGGCCgCGGCGCccgcGCGaGCCAc -3' miRNA: 3'- ugUUAGCUGG-GCCGCGuca-CGC-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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