Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18289 | 3' | -56.1 | NC_004681.1 | + | 17201 | 0.67 | 0.738729 |
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Target: 5'- gCGCCCGCGAucuUCUGGCccaucUGAgCCCa-- -3' miRNA: 3'- gGUGGGUGCU---AGGCCGu----ACU-GGGaag -5' |
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| 18289 | 3' | -56.1 | NC_004681.1 | + | 63195 | 0.67 | 0.738729 |
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Target: 5'- --gUCCACGAUCCGGCAcaGCUCa-- -3' miRNA: 3'- gguGGGUGCUAGGCCGUacUGGGaag -5' |
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| 18289 | 3' | -56.1 | NC_004681.1 | + | 31784 | 0.66 | 0.748824 |
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Target: 5'- ---aCCGCGcgCUGGCAcugcUGACCCUc- -3' miRNA: 3'- ggugGGUGCuaGGCCGU----ACUGGGAag -5' |
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| 18289 | 3' | -56.1 | NC_004681.1 | + | 12101 | 0.66 | 0.758808 |
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Target: 5'- uCCGCCCGCGcGUCUcGC-UGACCaaggUCg -3' miRNA: 3'- -GGUGGGUGC-UAGGcCGuACUGGga--AG- -5' |
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| 18289 | 3' | -56.1 | NC_004681.1 | + | 20520 | 0.66 | 0.768671 |
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Target: 5'- uCCACgCACGccUCCGcGCA-GACCCg-- -3' miRNA: 3'- -GGUGgGUGCu-AGGC-CGUaCUGGGaag -5' |
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| 18289 | 3' | -56.1 | NC_004681.1 | + | 48684 | 0.66 | 0.768671 |
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Target: 5'- gCCugCCucuGCGAg--GGCGUGGCCCa-- -3' miRNA: 3'- -GGugGG---UGCUaggCCGUACUGGGaag -5' |
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| 18289 | 3' | -56.1 | NC_004681.1 | + | 38142 | 0.66 | 0.787992 |
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Target: 5'- -aGCCC-CGAggCCGG-AUGGCCCUc- -3' miRNA: 3'- ggUGGGuGCUa-GGCCgUACUGGGAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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