Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18289 | 5' | -58.1 | NC_004681.1 | + | 52314 | 0.7 | 0.419739 |
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Target: 5'- aGGUCGgggauGGCgGGGCCGUucuuGGCCg -3' miRNA: 3'- aUCAGCaugguCCGgUCCGGCA----UCGG- -5' |
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| 18289 | 5' | -58.1 | NC_004681.1 | + | 52978 | 0.67 | 0.536145 |
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Target: 5'- -uGUCGccgcgGCuCGGGCacgcgggCAGGCCGaUGGCCa -3' miRNA: 3'- auCAGCa----UG-GUCCG-------GUCCGGC-AUCGG- -5' |
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| 18289 | 5' | -58.1 | NC_004681.1 | + | 53277 | 0.69 | 0.46594 |
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Target: 5'- gUAGUCGU-CCAaugucgcGGCCaugAGGaaguCCGUGGCCg -3' miRNA: 3'- -AUCAGCAuGGU-------CCGG---UCC----GGCAUCGG- -5' |
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| 18289 | 5' | -58.1 | NC_004681.1 | + | 60147 | 0.68 | 0.516674 |
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Target: 5'- cGGUCa-GCCGGuGCCAGGUgCGggAGCCg -3' miRNA: 3'- aUCAGcaUGGUC-CGGUCCG-GCa-UCGG- -5' |
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| 18289 | 5' | -58.1 | NC_004681.1 | + | 60550 | 0.66 | 0.653328 |
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Target: 5'- gUAGUCacGCCGcacGCCucGGGCCGcUGGCCg -3' miRNA: 3'- -AUCAGcaUGGUc--CGG--UCCGGC-AUCGG- -5' |
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| 18289 | 5' | -58.1 | NC_004681.1 | + | 65060 | 0.68 | 0.513625 |
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Target: 5'- gUAGUCGUGcggguaguagacguCCAGcuCCAGGCgCGUcGCCa -3' miRNA: 3'- -AUCAGCAU--------------GGUCc-GGUCCG-GCAuCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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