Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18289 | 5' | -58.1 | NC_004681.1 | + | 16326 | 0.71 | 0.333867 |
Target: 5'- gGGUCGUugggauuuccGCCgaAGGCguGGCCGUucugcuuGGCCu -3' miRNA: 3'- aUCAGCA----------UGG--UCCGguCCGGCA-------UCGG- -5' |
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18289 | 5' | -58.1 | NC_004681.1 | + | 13094 | 0.66 | 0.600119 |
Target: 5'- -cGUCGUcCgCGGuGCCAGcGgUGUAGCCg -3' miRNA: 3'- auCAGCAuG-GUC-CGGUC-CgGCAUCGG- -5' |
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18289 | 5' | -58.1 | NC_004681.1 | + | 12928 | 0.72 | 0.304332 |
Target: 5'- cUGGaCGUACCGGcGCCAauGGCCGUAcguGCUg -3' miRNA: 3'- -AUCaGCAUGGUC-CGGU--CCGGCAU---CGG- -5' |
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18289 | 5' | -58.1 | NC_004681.1 | + | 12247 | 0.66 | 0.600119 |
Target: 5'- gAGUCGggu--GGcCCGGuGCUGUGGCCg -3' miRNA: 3'- aUCAGCaugguCC-GGUC-CGGCAUCGG- -5' |
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18289 | 5' | -58.1 | NC_004681.1 | + | 10314 | 0.67 | 0.58952 |
Target: 5'- ---cCGgggaGCCAgGGCCAGG-UGUGGCCg -3' miRNA: 3'- aucaGCa---UGGU-CCGGUCCgGCAUCGG- -5' |
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18289 | 5' | -58.1 | NC_004681.1 | + | 4230 | 0.66 | 0.610743 |
Target: 5'- gGGUUGUugCGGGCCucagcGGCCu--GCUu -3' miRNA: 3'- aUCAGCAugGUCCGGu----CCGGcauCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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