Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18290 | 3' | -59.7 | NC_004681.1 | + | 49434 | 0.69 | 0.3939 |
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Target: 5'- cGcCCgcggCGCCGCCGU-GGcGGaCGGUGa -3' miRNA: 3'- aCaGGa---GCGGCGGUAuCCaCC-GCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 50871 | 0.66 | 0.566922 |
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Target: 5'- cUGcUCUCGCUugugaGCCA-GGGUGGCGGc- -3' miRNA: 3'- -ACaGGAGCGG-----CGGUaUCCACCGCCac -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 50930 | 0.69 | 0.368484 |
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Target: 5'- -cUCUUCGgCGagcUGGGUGGCGGUGg -3' miRNA: 3'- acAGGAGCgGCgguAUCCACCGCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 57805 | 0.7 | 0.360265 |
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Target: 5'- cG-CCgCGCCGCCG-AGGUGGCGa-- -3' miRNA: 3'- aCaGGaGCGGCGGUaUCCACCGCcac -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 57879 | 0.67 | 0.505972 |
|
Target: 5'- cGUggUCGCgGUCuccGGUGGCGGUGu -3' miRNA: 3'- aCAggAGCGgCGGuauCCACCGCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 65612 | 0.7 | 0.360265 |
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Target: 5'- cUG-CUUCGCCGCCAagUGGcGUGGCGa-- -3' miRNA: 3'- -ACaGGAGCGGCGGU--AUC-CACCGCcac -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 65719 | 0.69 | 0.411463 |
|
Target: 5'- uUGUCCUCGCCacGCCAcu--UGGCGGc- -3' miRNA: 3'- -ACAGGAGCGG--CGGUauccACCGCCac -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 66290 | 0.66 | 0.546346 |
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Target: 5'- gGUCCUUGCagCGCCAcaucuGGUacaugcccgcGGUGGUGg -3' miRNA: 3'- aCAGGAGCG--GCGGUau---CCA----------CCGCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 66913 | 0.67 | 0.515953 |
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Target: 5'- cUGUCCaUCGUCGUCGUugacucuGUGGCGGc- -3' miRNA: 3'- -ACAGG-AGCGGCGGUAuc-----CACCGCCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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