Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18290 | 3' | -59.7 | NC_004681.1 | + | 25021 | 0.66 | 0.577286 |
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Target: 5'- aGUCgaCGCCGCgCAgaaGGGUGGCa--- -3' miRNA: 3'- aCAGgaGCGGCG-GUa--UCCACCGccac -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 20662 | 0.67 | 0.515953 |
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Target: 5'- -uUCCgccagCGCCGCCAUuuGUgcGGCGGUu -3' miRNA: 3'- acAGGa----GCGGCGGUAucCA--CCGCCAc -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 19501 | 0.7 | 0.321119 |
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Target: 5'- cGUCCUCGUCGCCcguGUAGGUcuGGauGUa -3' miRNA: 3'- aCAGGAGCGGCGG---UAUCCA--CCgcCAc -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 18298 | 0.68 | 0.442404 |
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Target: 5'- aGUCCUCGaCUGgcggucagcagccuuCCGguucguccGGUGGCGGUGg -3' miRNA: 3'- aCAGGAGC-GGC---------------GGUau------CCACCGCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 14670 | 0.69 | 0.411463 |
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Target: 5'- gGUgCCgaaggcgacUGCgGCCGU-GGUGGCGGUGg -3' miRNA: 3'- aCA-GGa--------GCGgCGGUAuCCACCGCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 12492 | 0.67 | 0.515953 |
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Target: 5'- --aCCgacgagCGUCGCCGc-GGUGGUGGUGc -3' miRNA: 3'- acaGGa-----GCGGCGGUauCCACCGCCAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 10545 | 0.67 | 0.515953 |
|
Target: 5'- gGUCCUCcuaGCUaaGCCA-GGGUGGCGaUGa -3' miRNA: 3'- aCAGGAG---CGG--CGGUaUCCACCGCcAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 9420 | 0.67 | 0.49509 |
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Target: 5'- aUGUUCUUGCCGCCGUugaccGGGUugccguaGGCGuUGc -3' miRNA: 3'- -ACAGGAGCGGCGGUA-----UCCA-------CCGCcAC- -5' |
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| 18290 | 3' | -59.7 | NC_004681.1 | + | 3046 | 0.68 | 0.46693 |
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Target: 5'- gGUCCUCGCUGgugaacUCGgcgcGcGUGGCGGUGc -3' miRNA: 3'- aCAGGAGCGGC------GGUau--C-CACCGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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