Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18296 | 3' | -46.5 | NC_004681.1 | + | 29862 | 0.66 | 0.997629 |
Target: 5'- aUGCGgucaAGCUcggucacagaucGCAGUCUAGcaGGGCUu -3' miRNA: 3'- -ACGCaa--UCGA------------UGUCAGAUCaaCUUGA- -5' |
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18296 | 3' | -46.5 | NC_004681.1 | + | 62102 | 0.67 | 0.99427 |
Target: 5'- gGCGgcauccuGCUGCAGUCgg--UGGACg -3' miRNA: 3'- aCGCaau----CGAUGUCAGaucaACUUGa -5' |
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18296 | 3' | -46.5 | NC_004681.1 | + | 31110 | 0.67 | 0.992143 |
Target: 5'- cGCGUUGcGCUugcacCAGUCgUAGUUGuGCg -3' miRNA: 3'- aCGCAAU-CGAu----GUCAG-AUCAACuUGa -5' |
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18296 | 3' | -46.5 | NC_004681.1 | + | 57457 | 0.68 | 0.989432 |
Target: 5'- gGCGUUAGCgcGCGGUCccug-GAGCUc -3' miRNA: 3'- aCGCAAUCGa-UGUCAGaucaaCUUGA- -5' |
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18296 | 3' | -46.5 | NC_004681.1 | + | 26901 | 0.68 | 0.984061 |
Target: 5'- aGCGUUGGCcgccGCGGUgaAGUUGAc-- -3' miRNA: 3'- aCGCAAUCGa---UGUCAgaUCAACUuga -5' |
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18296 | 3' | -46.5 | NC_004681.1 | + | 30017 | 1.1 | 0.009042 |
Target: 5'- gUGCGUUAGCUACAGUCUAGUUGAACUa -3' miRNA: 3'- -ACGCAAUCGAUGUCAGAUCAACUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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