Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18297 | 3' | -52.2 | NC_004681.1 | + | 34970 | 0.66 | 0.916476 |
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Target: 5'- gAGCCGGuuGCCaUGGcUGUCGUACAUGu -3' miRNA: 3'- -UUGGCUu-CGGcACCaACAGUGUGUAC- -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 33942 | 0.66 | 0.8964 |
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Target: 5'- -uUUGAAGCCGUGGaUGUgACugAg- -3' miRNA: 3'- uuGGCUUCGGCACCaACAgUGugUac -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 10354 | 0.67 | 0.873876 |
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Target: 5'- cACCGGguuguAGCCGUGGguguagGUCACccGCAg- -3' miRNA: 3'- uUGGCU-----UCGGCACCaa----CAGUG--UGUac -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 12995 | 0.67 | 0.865848 |
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Target: 5'- cGGCaCGAAGCCGgugugGGUgugGUCACccGCAg- -3' miRNA: 3'- -UUG-GCUUCGGCa----CCAa--CAGUG--UGUac -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 69084 | 0.67 | 0.857572 |
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Target: 5'- gGAUCGAGGUCGU---UGUCGCACAc- -3' miRNA: 3'- -UUGGCUUCGGCAccaACAGUGUGUac -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 1324 | 0.68 | 0.837635 |
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Target: 5'- cGCCGAcaagggcugccucaAGCCGUGGgacGUC-UACAUGu -3' miRNA: 3'- uUGGCU--------------UCGGCACCaa-CAGuGUGUAC- -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 60530 | 0.69 | 0.763099 |
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Target: 5'- cGACCGAgacgGGCCGaagGGUaGUCACGCc-- -3' miRNA: 3'- -UUGGCU----UCGGCa--CCAaCAGUGUGuac -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 45110 | 0.7 | 0.69912 |
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Target: 5'- aGACCGggGCCGacGccGUCGCGCAg- -3' miRNA: 3'- -UUGGCuuCGGCacCaaCAGUGUGUac -5' |
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| 18297 | 3' | -52.2 | NC_004681.1 | + | 30197 | 1.06 | 0.004183 |
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Target: 5'- gAACCGAAGCCGUGGUUGUCACACAUGu -3' miRNA: 3'- -UUGGCUUCGGCACCAACAGUGUGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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