miRNA: 3'- -GCCCuuAGAGCG----------AGGUca--CCAaGUGc -5'
miRNA: 3'- -GCCCUUa---------GAGCGaGGUCACCa---AGUGC- -5'
miRNA: 3'- -GCCCUUAGAGCGAGGUCACCAAGUGC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18299 | 5' | -53.7 | NC_004681.1 | + | 54008 | 0.66 | 0.88485 |
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Target: 5'- cCGGGggUCUCGCauugcuggcuUCCAcgagGGUcCGCc -3' miRNA: 3'- -GCCCuuAGAGCG----------AGGUca--CCAaGUGc -5' |
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| 18299 | 5' | -53.7 | NC_004681.1 | + | 63206 | 0.67 | 0.827884 |
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Target: 5'- uCGGGAAgagguucggcUUCGC-CCGGUGGgacgUUACGa -3' miRNA: 3'- -GCCCUUa---------GAGCGaGGUCACCa---AGUGC- -5' |
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| 18299 | 5' | -53.7 | NC_004681.1 | + | 30741 | 1.09 | 0.002431 |
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Target: 5'- gCGGGAAUCUCGCUCCAGUGGUUCACGu -3' miRNA: 3'- -GCCCUUAGAGCGAGGUCACCAAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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