Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18301 | 3' | -57.7 | NC_004681.1 | + | 39933 | 0.71 | 0.399193 |
Target: 5'- cGCgGGCGCggugCGCGCGGUGCGGu -3' miRNA: 3'- uCGaCUGUGggaaGCGCGCCAUGCCc -5' |
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18301 | 3' | -57.7 | NC_004681.1 | + | 13127 | 0.71 | 0.390508 |
Target: 5'- cGCUGcccgccacCGCCCUUCGUggcacgGCgGGUACGGGc -3' miRNA: 3'- uCGACu-------GUGGGAAGCG------CG-CCAUGCCC- -5' |
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18301 | 3' | -57.7 | NC_004681.1 | + | 50382 | 0.71 | 0.381948 |
Target: 5'- cGCgggGACgugGCCCUUCuuGCGGUgggcGCGGGa -3' miRNA: 3'- uCGa--CUG---UGGGAAGcgCGCCA----UGCCC- -5' |
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18301 | 3' | -57.7 | NC_004681.1 | + | 24917 | 0.73 | 0.325615 |
Target: 5'- cAGCUGcCACCCUUCuGCGCGGcguCGa- -3' miRNA: 3'- -UCGACuGUGGGAAG-CGCGCCau-GCcc -5' |
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18301 | 3' | -57.7 | NC_004681.1 | + | 33078 | 1.09 | 0.000922 |
Target: 5'- uAGCUGACACCCUUCGCGCGGUACGGGu -3' miRNA: 3'- -UCGACUGUGGGAAGCGCGCCAUGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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