Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18303 | 3' | -56.4 | NC_004681.1 | + | 69218 | 0.72 | 0.412414 |
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Target: 5'- aGGGAGgccaGGGCGCgggCGA--GGCGGCg -3' miRNA: 3'- gCCCUCac--UUCGCGa--GCUucUCGCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 66873 | 0.72 | 0.412414 |
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Target: 5'- aGGGAGUGcucAGCG-UCGAuguaGGCGGCg -3' miRNA: 3'- gCCCUCACu--UCGCgAGCUuc--UCGCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 22605 | 0.71 | 0.448577 |
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Target: 5'- gCGGGAGUucccgauacguccGAGGCGC-CGAugauGGUGGCg -3' miRNA: 3'- -GCCCUCA-------------CUUCGCGaGCUuc--UCGCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 62303 | 0.66 | 0.758808 |
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Target: 5'- gCGGGAugGAAGCGCacCGAcuGGUGGUu -3' miRNA: 3'- -GCCCUcaCUUCGCGa-GCUucUCGCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 57999 | 0.66 | 0.749828 |
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Target: 5'- aGGGAG-GAGGCGCUCuaccaacgccucaaGAggcucaagcagacggAGgaaucGGCGGCa -3' miRNA: 3'- gCCCUCaCUUCGCGAG--------------CU---------------UC-----UCGCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 31000 | 0.68 | 0.659466 |
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Target: 5'- uGGGcgcacaacuacgacuGGUGcAAGCGCaacgCGGAcAGCGGCa -3' miRNA: 3'- gCCC---------------UCAC-UUCGCGa---GCUUcUCGCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 60905 | 0.68 | 0.655218 |
|
Target: 5'- uGGGGGUGuAGUGCUgGAccccuucgcgGGcAGCGGa -3' miRNA: 3'- gCCCUCACuUCGCGAgCU----------UC-UCGCCg -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 40547 | 0.68 | 0.606289 |
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Target: 5'- aGGGAGUGGAGCcacucaccgagcgucGC-CGAGGcaaaCGGCg -3' miRNA: 3'- gCCCUCACUUCG---------------CGaGCUUCuc--GCCG- -5' |
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| 18303 | 3' | -56.4 | NC_004681.1 | + | 48784 | 0.7 | 0.518577 |
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Target: 5'- -uGGAGUGAGGCcgUCGAGGGcGCGGg -3' miRNA: 3'- gcCCUCACUUCGcgAGCUUCU-CGCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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