Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18303 | 5' | -55.3 | NC_004681.1 | + | 16243 | 0.68 | 0.645381 |
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Target: 5'- -cGACGG----GAUGGCCgccagCGCCGCg -3' miRNA: 3'- aaCUGCCagagCUACCGGaa---GUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 20019 | 0.69 | 0.634572 |
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Target: 5'- gUUGAgcaGGcCcgCGGUGGCCUUCauGCCGUg -3' miRNA: 3'- -AACUg--CCaGa-GCUACCGGAAG--UGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 16715 | 0.69 | 0.634572 |
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Target: 5'- aUGACGGg--CGGUGGCagcgauggCACCGUc -3' miRNA: 3'- aACUGCCagaGCUACCGgaa-----GUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 30361 | 0.7 | 0.569986 |
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Target: 5'- -cGGUGGUCUCGAcgccguaccGGCCgcCGCCGCu -3' miRNA: 3'- aaCUGCCAGAGCUa--------CCGGaaGUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 39251 | 0.7 | 0.531962 |
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Target: 5'- aUGcCGGUCUCGGUGaacagcacgaugagcGCCUugUCGCgGCg -3' miRNA: 3'- aACuGCCAGAGCUAC---------------CGGA--AGUGgCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 54510 | 0.71 | 0.466891 |
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Target: 5'- -aGAgcUGGUCccaggaUCGGUGGCCcgagaUCACCGCa -3' miRNA: 3'- aaCU--GCCAG------AGCUACCGGa----AGUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 68994 | 0.72 | 0.437825 |
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Target: 5'- -cGAUGGUCagcgccUCGAUGGCgUcgGCCGCg -3' miRNA: 3'- aaCUGCCAG------AGCUACCGgAagUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 14748 | 0.74 | 0.323447 |
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Target: 5'- --cACGGUCUCGAaGGCCgguuuccgugaCGCCGCg -3' miRNA: 3'- aacUGCCAGAGCUaCCGGaa---------GUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 16023 | 0.78 | 0.201379 |
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Target: 5'- -cGAUGGcCUgGAUGGUCUUgACCGCg -3' miRNA: 3'- aaCUGCCaGAgCUACCGGAAgUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 33624 | 1.08 | 0.001611 |
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Target: 5'- cUUGACGGUCUCGAUGGCCUUCACCGCc -3' miRNA: 3'- -AACUGCCAGAGCUACCGGAAGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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