Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18303 | 5' | -55.3 | NC_004681.1 | + | 41783 | 0.66 | 0.809697 |
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Target: 5'- ----aGG-UUCGAcGGCCUUCgcGCCGCa -3' miRNA: 3'- aacugCCaGAGCUaCCGGAAG--UGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 45096 | 0.67 | 0.696927 |
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Target: 5'- --cGCGGUCUCGcucaagaccggGGCCgaCGCCGUc -3' miRNA: 3'- aacUGCCAGAGCua---------CCGGaaGUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 49202 | 0.66 | 0.771248 |
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Target: 5'- -aGAUGGUCUCGGaacGGCUgaagUgACcCGCg -3' miRNA: 3'- aaCUGCCAGAGCUa--CCGGa---AgUG-GCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 54353 | 0.66 | 0.790791 |
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Target: 5'- -cGGCGGUCgUGAUgcGGUCUUCGucCUGCu -3' miRNA: 3'- aaCUGCCAGaGCUA--CCGGAAGU--GGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 54510 | 0.71 | 0.466891 |
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Target: 5'- -aGAgcUGGUCccaggaUCGGUGGCCcgagaUCACCGCa -3' miRNA: 3'- aaCU--GCCAG------AGCUACCGGa----AGUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 54849 | 0.67 | 0.699053 |
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Target: 5'- aUGACGaugacCUCGggGGCCUggUCAUCGUc -3' miRNA: 3'- aACUGCca---GAGCuaCCGGA--AGUGGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 56210 | 0.66 | 0.809697 |
|
Target: 5'- -aGcCGGUCUCGuucgGGUCcUCGuuGCg -3' miRNA: 3'- aaCuGCCAGAGCua--CCGGaAGUggCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 57873 | 0.66 | 0.771248 |
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Target: 5'- --cGCGGUCUcCGGUGGCggugUCGCCu- -3' miRNA: 3'- aacUGCCAGA-GCUACCGga--AGUGGcg -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 60433 | 0.66 | 0.761263 |
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Target: 5'- -cGACGGgCUcaaaCGAUGGUUUUUAgCGCg -3' miRNA: 3'- aaCUGCCaGA----GCUACCGGAAGUgGCG- -5' |
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| 18303 | 5' | -55.3 | NC_004681.1 | + | 68994 | 0.72 | 0.437825 |
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Target: 5'- -cGAUGGUCagcgccUCGAUGGCgUcgGCCGCg -3' miRNA: 3'- aaCUGCCAG------AGCUACCGgAagUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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