Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18308 | 3' | -53 | NC_004681.1 | + | 9075 | 0.68 | 0.778819 |
Target: 5'- gUG-GCGCCGAcAgCGAGGAggcCGAGGAg -3' miRNA: 3'- gACaCGCGGCUuUgGCUUCU---GCUCUU- -5' |
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18308 | 3' | -53 | NC_004681.1 | + | 3750 | 0.72 | 0.585393 |
Target: 5'- uCUGgGCGgCGAcgUCGAAGGCGAGGg -3' miRNA: 3'- -GACaCGCgGCUuuGGCUUCUGCUCUu -5' |
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18308 | 3' | -53 | NC_004681.1 | + | 730 | 0.67 | 0.817431 |
Target: 5'- ---gGCGUCGAucaccgGGCCGAAGGCGcGGAGg -3' miRNA: 3'- gacaCGCGGCU------UUGGCUUCUGC-UCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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