Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18309 | 3' | -54.3 | NC_004681.1 | + | 56049 | 0.66 | 0.849275 |
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Target: 5'- uGGAGAcgcagcuGUCCGUGGACUAcgCGcACUa -3' miRNA: 3'- -CCUCUac-----CAGGCACCUGGUa-GC-UGAg -5' |
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| 18309 | 3' | -54.3 | NC_004681.1 | + | 36374 | 0.66 | 0.840839 |
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Target: 5'- cGGAgucGAUGGUCCacGGACCAUCuccGAUa- -3' miRNA: 3'- -CCU---CUACCAGGcaCCUGGUAG---CUGag -5' |
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| 18309 | 3' | -54.3 | NC_004681.1 | + | 53254 | 0.68 | 0.746504 |
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Target: 5'- uGAGGaaGUCCGUGG-CCGggUGACUCa -3' miRNA: 3'- cCUCUacCAGGCACCuGGUa-GCUGAG- -5' |
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| 18309 | 3' | -54.3 | NC_004681.1 | + | 29580 | 0.69 | 0.725978 |
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Target: 5'- cGAGGUGGUcCCGgugagGGACagggugacCGACUCa -3' miRNA: 3'- cCUCUACCA-GGCa----CCUGgua-----GCUGAG- -5' |
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| 18309 | 3' | -54.3 | NC_004681.1 | + | 20212 | 0.69 | 0.683942 |
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Target: 5'- aGGAGG-GGUCCGaacGuGAUCGUgGGCUCg -3' miRNA: 3'- -CCUCUaCCAGGCa--C-CUGGUAgCUGAG- -5' |
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| 18309 | 3' | -54.3 | NC_004681.1 | + | 36477 | 1.11 | 0.001477 |
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Target: 5'- cGGAGAUGGUCCGUGGACCAUCGACUCc -3' miRNA: 3'- -CCUCUACCAGGCACCUGGUAGCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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