Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18311 | 5' | -56.5 | NC_004681.1 | + | 43781 | 0.66 | 0.732743 |
Target: 5'- cGGGGUGUguUgGAGggGuugaUCGGcGGGc -3' miRNA: 3'- uUCCCACAguAgCUCuuC----AGCC-CCCa -5' |
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18311 | 5' | -56.5 | NC_004681.1 | + | 7897 | 0.66 | 0.732743 |
Target: 5'- -cGGG-GagGUCG-GGAGUCuGGGGGUu -3' miRNA: 3'- uuCCCaCagUAGCuCUUCAG-CCCCCA- -5' |
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18311 | 5' | -56.5 | NC_004681.1 | + | 51494 | 0.66 | 0.701418 |
Target: 5'- gGAGGGcacGUUcugcugcauGUCGAGGAGcUGGGGGg -3' miRNA: 3'- -UUCCCa--CAG---------UAGCUCUUCaGCCCCCa -5' |
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18311 | 5' | -56.5 | NC_004681.1 | + | 54700 | 0.66 | 0.690834 |
Target: 5'- cAGGGUGcCGUCGAuguccacgauGAuugCGGGGGg -3' miRNA: 3'- uUCCCACaGUAGCU----------CUucaGCCCCCa -5' |
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18311 | 5' | -56.5 | NC_004681.1 | + | 34496 | 0.69 | 0.541365 |
Target: 5'- cAAGGGUG-CAgaguuugaUCGGGAucGUCuGGGGGUg -3' miRNA: 3'- -UUCCCACaGU--------AGCUCUu-CAG-CCCCCA- -5' |
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18311 | 5' | -56.5 | NC_004681.1 | + | 30976 | 0.71 | 0.431686 |
Target: 5'- cAGGGUGUCG-CGGGGcuccagcgGGUCGGcGGGc -3' miRNA: 3'- uUCCCACAGUaGCUCU--------UCAGCC-CCCa -5' |
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18311 | 5' | -56.5 | NC_004681.1 | + | 37621 | 1.05 | 0.001884 |
Target: 5'- cAAGGGUGUCAUCGAGAAGUCGGGGGUg -3' miRNA: 3'- -UUCCCACAGUAGCUCUUCAGCCCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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