Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18313 | 5' | -54.6 | NC_004681.1 | + | 38985 | 0.67 | 0.760549 |
Target: 5'- --aGCAGGCUGGCCuucuccAGUggcGCCa -3' miRNA: 3'- caaCGUCCGACCGGuacc--UCAac-UGG- -5' |
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18313 | 5' | -54.6 | NC_004681.1 | + | 42241 | 0.67 | 0.760549 |
Target: 5'- cGUUGgccucuauCAGGCUGG-CGUGGAccagGUUGGCa -3' miRNA: 3'- -CAAC--------GUCCGACCgGUACCU----CAACUGg -5' |
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18313 | 5' | -54.6 | NC_004681.1 | + | 74268 | 0.67 | 0.770673 |
Target: 5'- cGUUGC--GCUGGCCAccu-GUUGGCCc -3' miRNA: 3'- -CAACGucCGACCGGUaccuCAACUGG- -5' |
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18313 | 5' | -54.6 | NC_004681.1 | + | 8950 | 0.66 | 0.780655 |
Target: 5'- --aGCAGGC-GGCCcUGGAcgaaGUUGAa- -3' miRNA: 3'- caaCGUCCGaCCGGuACCU----CAACUgg -5' |
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18313 | 5' | -54.6 | NC_004681.1 | + | 40091 | 0.66 | 0.780655 |
Target: 5'- --aGCGGcGCUGGUucaaggccgagCcgGuGAGUUGACCc -3' miRNA: 3'- caaCGUC-CGACCG-----------GuaC-CUCAACUGG- -5' |
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18313 | 5' | -54.6 | NC_004681.1 | + | 62766 | 0.66 | 0.818951 |
Target: 5'- --cGaCAGGCUGaCCGccGAGUUGGCUa -3' miRNA: 3'- caaC-GUCCGACcGGUacCUCAACUGG- -5' |
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18313 | 5' | -54.6 | NC_004681.1 | + | 62600 | 0.66 | 0.818951 |
Target: 5'- -gUGCccauGGCcugGGCCAUgaucaGGAGUUcguaGACCa -3' miRNA: 3'- caACGu---CCGa--CCGGUA-----CCUCAA----CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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