Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18313 | 5' | -54.6 | NC_004681.1 | + | 21343 | 0.72 | 0.462604 |
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Target: 5'- ---aCAGGCUGGCCGUGaGAuGcaGACCg -3' miRNA: 3'- caacGUCCGACCGGUAC-CU-CaaCUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 16616 | 0.67 | 0.729442 |
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Target: 5'- -cUGCAGGCc-GCgAUGGAGgcGGCg -3' miRNA: 3'- caACGUCCGacCGgUACCUCaaCUGg -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 16500 | 0.7 | 0.555967 |
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Target: 5'- --cGCAuGGcCUGGUCAcccuUGGAGgUGGCCg -3' miRNA: 3'- caaCGU-CC-GACCGGU----ACCUCaACUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 8950 | 0.66 | 0.780655 |
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Target: 5'- --aGCAGGC-GGCCcUGGAcgaaGUUGAa- -3' miRNA: 3'- caaCGUCCGaCCGGuACCU----CAACUgg -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 6360 | 0.71 | 0.534631 |
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Target: 5'- cGUU-CGGGgUGGCgAcaccGGAGUUGACCa -3' miRNA: 3'- -CAAcGUCCgACCGgUa---CCUCAACUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 2496 | 0.72 | 0.452725 |
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Target: 5'- cUUGCGGGUguacUGGCCuuUGGuGUgGACCa -3' miRNA: 3'- cAACGUCCG----ACCGGu-ACCuCAaCUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 2024 | 0.68 | 0.697468 |
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Target: 5'- --gGcCAGG-UGGUCGUGGAGggcGGCCg -3' miRNA: 3'- caaC-GUCCgACCGGUACCUCaa-CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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