Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18313 | 5' | -54.6 | NC_004681.1 | + | 2496 | 0.72 | 0.452725 |
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Target: 5'- cUUGCGGGUguacUGGCCuuUGGuGUgGACCa -3' miRNA: 3'- cAACGUCCG----ACCGGu-ACCuCAaCUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 62766 | 0.66 | 0.818951 |
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Target: 5'- --cGaCAGGCUGaCCGccGAGUUGGCUa -3' miRNA: 3'- caaC-GUCCGACcGGUacCUCAACUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 16616 | 0.67 | 0.729442 |
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Target: 5'- -cUGCAGGCc-GCgAUGGAGgcGGCg -3' miRNA: 3'- caACGUCCGacCGgUACCUCaaCUGg -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 66864 | 0.67 | 0.718866 |
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Target: 5'- -cUGCGaGCaGGCCcUGGAgaucGUUGACCu -3' miRNA: 3'- caACGUcCGaCCGGuACCU----CAACUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 2024 | 0.68 | 0.697468 |
|
Target: 5'- --gGcCAGG-UGGUCGUGGAGggcGGCCg -3' miRNA: 3'- caaC-GUCCgACCGGUACCUCaa-CUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 30160 | 0.7 | 0.577543 |
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Target: 5'- --gGCGGcGC-GGCCGcGGAGUgGACCu -3' miRNA: 3'- caaCGUC-CGaCCGGUaCCUCAaCUGG- -5' |
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| 18313 | 5' | -54.6 | NC_004681.1 | + | 33462 | 0.78 | 0.19154 |
|
Target: 5'- cGUUGCGGGCcagUGGCCGUGGcugauccuGG-UGGCCg -3' miRNA: 3'- -CAACGUCCG---ACCGGUACC--------UCaACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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