Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18314 | 5' | -54.3 | NC_004681.1 | + | 49581 | 0.69 | 0.705956 |
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Target: 5'- cCCCggcGACUGGGCAGUAgGGCUUg-- -3' miRNA: 3'- aGGGa--CUGGCCCGUUAUgUUGGAgcu -5' |
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| 18314 | 5' | -54.3 | NC_004681.1 | + | 17850 | 0.69 | 0.69533 |
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Target: 5'- gCCCUGcuCCuGGCccuugGCGACCUCGGu -3' miRNA: 3'- aGGGACu-GGcCCGuua--UGUUGGAGCU- -5' |
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| 18314 | 5' | -54.3 | NC_004681.1 | + | 39775 | 0.69 | 0.67391 |
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Target: 5'- cUUuuUGugCGGGCAAUuGCuGCCUCGc -3' miRNA: 3'- -AGggACugGCCCGUUA-UGuUGGAGCu -5' |
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| 18314 | 5' | -54.3 | NC_004681.1 | + | 66542 | 0.71 | 0.555436 |
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Target: 5'- gCCCUGGCCGaGGuCGAUAaacGCUUCGGc -3' miRNA: 3'- aGGGACUGGC-CC-GUUAUgu-UGGAGCU- -5' |
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| 18314 | 5' | -54.3 | NC_004681.1 | + | 63431 | 0.74 | 0.415438 |
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Target: 5'- uUCCCUGGCCaccaGGCuuc-CAGCCUCGGc -3' miRNA: 3'- -AGGGACUGGc---CCGuuauGUUGGAGCU- -5' |
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| 18314 | 5' | -54.3 | NC_004681.1 | + | 40627 | 1.1 | 0.001726 |
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Target: 5'- cUCCCUGACCGGGCAAUACAACCUCGAg -3' miRNA: 3'- -AGGGACUGGCCCGUUAUGUUGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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