Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18317 | 3' | -57.1 | NC_004681.1 | + | 3049 | 0.66 | 0.71285 |
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Target: 5'- gCGCCAa-GGGCcuggUGGcAGUCCUGCGc -3' miRNA: 3'- -GCGGUagUCCGcaa-GCC-UCAGGACGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 62435 | 0.66 | 0.711811 |
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Target: 5'- aGCCGUaacgcaGGGCcucgUCGGAGUCCaaccaccagucggUGCGc -3' miRNA: 3'- gCGGUAg-----UCCGca--AGCCUCAGG-------------ACGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 46733 | 0.66 | 0.691936 |
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Target: 5'- aCGCC-UCAaagacagugGGCGUgugcggggCGGGGUCCUcgGCGa -3' miRNA: 3'- -GCGGuAGU---------CCGCAa-------GCCUCAGGA--CGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 66896 | 0.67 | 0.638878 |
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Target: 5'- gCGCCuu--GGCGUacUCGGGGUCCagggagUGCu -3' miRNA: 3'- -GCGGuaguCCGCA--AGCCUCAGG------ACGu -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 42056 | 0.67 | 0.628211 |
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Target: 5'- gCGCCGguuaagCAGGCGccauugaggUUGGcGUCUUGCAg -3' miRNA: 3'- -GCGGUa-----GUCCGCa--------AGCCuCAGGACGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 24755 | 0.69 | 0.523067 |
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Target: 5'- aGC--UCGGGCGgguugUCGGGGUCC-GCGg -3' miRNA: 3'- gCGguAGUCCGCa----AGCCUCAGGaCGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 6463 | 0.7 | 0.472948 |
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Target: 5'- gCGCCAUCGGGCGUggCGcccaAGggCUGCAg -3' miRNA: 3'- -GCGGUAGUCCGCAa-GCc---UCagGACGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 5335 | 0.72 | 0.370519 |
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Target: 5'- uGCCcacagCGGGCGcguaguaguaGGAGUCCUGCAu -3' miRNA: 3'- gCGGua---GUCCGCaag-------CCUCAGGACGU- -5' |
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| 18317 | 3' | -57.1 | NC_004681.1 | + | 45919 | 1.09 | 0.000939 |
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Target: 5'- aCGCCAUCAGGCGUUCGGAGUCCUGCAg -3' miRNA: 3'- -GCGGUAGUCCGCAAGCCUCAGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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