Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18322 | 3' | -58.4 | NC_004681.1 | + | 36889 | 0.66 | 0.607805 |
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Target: 5'- aCUGCCUaGucAGCggGGGGCGGGCCAg -3' miRNA: 3'- aGGCGGGcCu-UCGagUCCCGUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 17597 | 0.66 | 0.618413 |
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Target: 5'- gCCGUgguaUCGGcGGCUCGGGcGCGGACg- -3' miRNA: 3'- aGGCG----GGCCuUCGAGUCC-CGUUUGgu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 27456 | 0.66 | 0.618413 |
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Target: 5'- uUCCGCCguugaGGGucAGCUCgaaugGGGGCGguguGACCu -3' miRNA: 3'- -AGGCGGg----CCU--UCGAG-----UCCCGU----UUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 3245 | 0.66 | 0.618413 |
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Target: 5'- gUCCaucagGCCCuGGAGG---AGGGCGAGCCGc -3' miRNA: 3'- -AGG-----CGGG-CCUUCgagUCCCGUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 63279 | 0.66 | 0.618413 |
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Target: 5'- cUCCGCCgCGGAca-UCAGGGUgucGAGCgGg -3' miRNA: 3'- -AGGCGG-GCCUucgAGUCCCG---UUUGgU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 19017 | 0.66 | 0.629032 |
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Target: 5'- cCCGCgaCGGGAGCcUGGGGaCcGGCCAg -3' miRNA: 3'- aGGCGg-GCCUUCGaGUCCC-GuUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 59463 | 0.66 | 0.639654 |
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Target: 5'- -gCGCaUCGGGAGCagAGGGUggGgCCAg -3' miRNA: 3'- agGCG-GGCCUUCGagUCCCGuuU-GGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 30337 | 0.66 | 0.639654 |
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Target: 5'- uUCgGCUCGGggGCUUucgcauuGGGgGAACa- -3' miRNA: 3'- -AGgCGGGCCuuCGAGu------CCCgUUUGgu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 25817 | 0.66 | 0.639654 |
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Target: 5'- aCCGCCaccgCGGu-GCUCAuGGCAAuCCAg -3' miRNA: 3'- aGGCGG----GCCuuCGAGUcCCGUUuGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 16605 | 0.66 | 0.639654 |
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Target: 5'- cCCGCCaucuGGGAGUUCcaguugccGGGGCGcggcGCCGa -3' miRNA: 3'- aGGCGGg---CCUUCGAG--------UCCCGUu---UGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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