Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18322 | 3' | -58.4 | NC_004681.1 | + | 44796 | 0.69 | 0.44551 |
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Target: 5'- aCCGCCCuGGAGGU---GGGCAaGACCu -3' miRNA: 3'- aGGCGGG-CCUUCGaguCCCGU-UUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 14443 | 0.69 | 0.44551 |
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Target: 5'- gCCGCCCGcGAGGC--GGaGGCGAacGCCGc -3' miRNA: 3'- aGGCGGGC-CUUCGagUC-CCGUU--UGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 40136 | 0.69 | 0.436125 |
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Target: 5'- aCCGCCCGGGA---CAGG-CAGGCCGc -3' miRNA: 3'- aGGCGGGCCUUcgaGUCCcGUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 48039 | 0.7 | 0.399762 |
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Target: 5'- cCCGCCUGGggGCUCuGGau--GCCu -3' miRNA: 3'- aGGCGGGCCuuCGAGuCCcguuUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 67367 | 0.7 | 0.390979 |
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Target: 5'- -gCGCCUgguucaaguacgGGGAcaagaacgucGCUCAGGGCAAGCCc -3' miRNA: 3'- agGCGGG------------CCUU----------CGAGUCCCGUUUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 22294 | 0.7 | 0.373794 |
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Target: 5'- cCUGCCCGucauGGCgCAGGGUGAGCCc -3' miRNA: 3'- aGGCGGGCcu--UCGaGUCCCGUUUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 49335 | 0.72 | 0.310324 |
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Target: 5'- gUCCGCCaCGGcGGCgccgCGGGcGCuGACCAc -3' miRNA: 3'- -AGGCGG-GCCuUCGa---GUCC-CGuUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 28483 | 0.72 | 0.288731 |
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Target: 5'- cCCGCCCGGGgcugGGCUCAcacucaccccGGGCAcccCCGc -3' miRNA: 3'- aGGCGGGCCU----UCGAGU----------CCCGUuu-GGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 63934 | 0.75 | 0.17868 |
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Target: 5'- cCCGCCCGGAuuuGaacCGGGGaCGAACCAa -3' miRNA: 3'- aGGCGGGCCUu--Cga-GUCCC-GUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 52936 | 1.09 | 0.000706 |
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Target: 5'- uUCCGCCCGGAAGCUCAGGGCAAACCAg -3' miRNA: 3'- -AGGCGGGCCUUCGAGUCCCGUUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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