Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18322 | 3' | -58.4 | NC_004681.1 | + | 49335 | 0.72 | 0.310324 |
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Target: 5'- gUCCGCCaCGGcGGCgccgCGGGcGCuGACCAc -3' miRNA: 3'- -AGGCGG-GCCuUCGa---GUCC-CGuUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 49647 | 0.66 | 0.597217 |
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Target: 5'- cCUGCCCGcGGgggaaGGC-CAGGGCGAugUc -3' miRNA: 3'- aGGCGGGC-CU-----UCGaGUCCCGUUugGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 52936 | 1.09 | 0.000706 |
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Target: 5'- uUCCGCCCGGAAGCUCAGGGCAAACCAg -3' miRNA: 3'- -AGGCGGGCCUUCGAGUCCCGUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 52977 | 0.67 | 0.555216 |
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Target: 5'- gUCGCCgCGGcucGGGCaCGcGGGCAGGCCGa -3' miRNA: 3'- aGGCGG-GCC---UUCGaGU-CCCGUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 59463 | 0.66 | 0.639654 |
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Target: 5'- -gCGCaUCGGGAGCagAGGGUggGgCCAg -3' miRNA: 3'- agGCG-GGCCUUCGagUCCCGuuU-GGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 62443 | 0.66 | 0.607805 |
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Target: 5'- gCCGCCCaGGAaacccgaauGGCcgaGGGGCAcGACCc -3' miRNA: 3'- aGGCGGG-CCU---------UCGag-UCCCGU-UUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 62676 | 0.67 | 0.53453 |
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Target: 5'- cUCCGCCuCGGcgcGCUCccGGCGuuCCAu -3' miRNA: 3'- -AGGCGG-GCCuu-CGAGucCCGUuuGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 63279 | 0.66 | 0.618413 |
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Target: 5'- cUCCGCCgCGGAca-UCAGGGUgucGAGCgGg -3' miRNA: 3'- -AGGCGG-GCCUucgAGUCCCG---UUUGgU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 63934 | 0.75 | 0.17868 |
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Target: 5'- cCCGCCCGGAuuuGaacCGGGGaCGAACCAa -3' miRNA: 3'- aGGCGGGCCUu--Cga-GUCCC-GUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 67367 | 0.7 | 0.390979 |
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Target: 5'- -gCGCCUgguucaaguacgGGGAcaagaacgucGCUCAGGGCAAGCCc -3' miRNA: 3'- agGCGGG------------CCUU----------CGAGUCCCGUUUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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