Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18322 | 3' | -58.4 | NC_004681.1 | + | 25817 | 0.66 | 0.639654 |
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Target: 5'- aCCGCCaccgCGGu-GCUCAuGGCAAuCCAg -3' miRNA: 3'- aGGCGG----GCCuuCGAGUcCCGUUuGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 22294 | 0.7 | 0.373794 |
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Target: 5'- cCUGCCCGucauGGCgCAGGGUGAGCCc -3' miRNA: 3'- aGGCGGGCcu--UCGaGUCCCGUUUGGu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 21950 | 0.67 | 0.524288 |
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Target: 5'- -aUGCCCG--AGCgaAGGGCGAACCAu -3' miRNA: 3'- agGCGGGCcuUCGagUCCCGUUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 19017 | 0.66 | 0.629032 |
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Target: 5'- cCCGCgaCGGGAGCcUGGGGaCcGGCCAg -3' miRNA: 3'- aGGCGg-GCCUUCGaGUCCC-GuUUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 17597 | 0.66 | 0.618413 |
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Target: 5'- gCCGUgguaUCGGcGGCUCGGGcGCGGACg- -3' miRNA: 3'- aGGCG----GGCCuUCGAGUCC-CGUUUGgu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 16605 | 0.66 | 0.639654 |
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Target: 5'- cCCGCCaucuGGGAGUUCcaguugccGGGGCGcggcGCCGa -3' miRNA: 3'- aGGCGGg---CCUUCGAG--------UCCCGUu---UGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 14443 | 0.69 | 0.44551 |
|
Target: 5'- gCCGCCCGcGAGGC--GGaGGCGAacGCCGc -3' miRNA: 3'- aGGCGGGC-CUUCGagUC-CCGUU--UGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 6784 | 0.66 | 0.585602 |
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Target: 5'- uUCCGCUCGGccagugcGAGCUgCAcGGCGAGCa- -3' miRNA: 3'- -AGGCGGGCC-------UUCGA-GUcCCGUUUGgu -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 3867 | 0.66 | 0.596159 |
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Target: 5'- cUCCGaaCCGGuaugGGCUCAGGuGCGcgcgugcAACCAc -3' miRNA: 3'- -AGGCg-GGCCu---UCGAGUCC-CGU-------UUGGU- -5' |
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| 18322 | 3' | -58.4 | NC_004681.1 | + | 3245 | 0.66 | 0.618413 |
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Target: 5'- gUCCaucagGCCCuGGAGG---AGGGCGAGCCGc -3' miRNA: 3'- -AGG-----CGGG-CCUUCgagUCCCGUUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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