Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18327 | 3' | -58.8 | NC_004681.1 | + | 2927 | 0.69 | 0.451676 |
Target: 5'- uGGGcGaCGGCACCgccacgcgcGCCGAguucaccAGCgagGACCCc -3' miRNA: 3'- -CCC-CaGCCGUGG---------UGGCU-------UCGa--CUGGG- -5' |
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18327 | 3' | -58.8 | NC_004681.1 | + | 1664 | 0.66 | 0.649677 |
Target: 5'- uGGGGcCGucuuccucuccccCGCCGCCcagcugaAGGCUGGCCCg -3' miRNA: 3'- -CCCCaGCc------------GUGGUGGc------UUCGACUGGG- -5' |
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18327 | 3' | -58.8 | NC_004681.1 | + | 658 | 0.69 | 0.452608 |
Target: 5'- uGGGGaugCGGCcguCACUGGAGCUuguGCCCg -3' miRNA: 3'- -CCCCa--GCCGug-GUGGCUUCGAc--UGGG- -5' |
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18327 | 3' | -58.8 | NC_004681.1 | + | 475 | 0.73 | 0.255209 |
Target: 5'- uGGGGa-GGCGCCugGCCGAgaugcugggcgucaAGCUGgACCCg -3' miRNA: 3'- -CCCCagCCGUGG--UGGCU--------------UCGAC-UGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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