Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18329 | 5' | -50 | NC_004681.1 | + | 15855 | 0.66 | 0.976526 |
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Target: 5'- aGGucUCGCCCAUCuGGAUgccCAAgGAGu -3' miRNA: 3'- gCUuaAGCGGGUAG-CCUA---GUUgCUCc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 75161 | 0.66 | 0.976526 |
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Target: 5'- cCGGuaUCGCCUAcCGGGUCGAgGAc- -3' miRNA: 3'- -GCUuaAGCGGGUaGCCUAGUUgCUcc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 6985 | 0.66 | 0.976526 |
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Target: 5'- aCGAAgcagGCCCAggauguUCGGAcccUCGuggACGAGGg -3' miRNA: 3'- -GCUUaag-CGGGU------AGCCU---AGU---UGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 35482 | 0.66 | 0.97905 |
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Target: 5'- gGAAUcCGgCCAccgguggcUUGGAcgCAGCGAGGa -3' miRNA: 3'- gCUUAaGCgGGU--------AGCCUa-GUUGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 32868 | 0.66 | 0.983282 |
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Target: 5'- aCGAGcg-GCCCAgCGGAUUgccgaccgggccgAACGAGGc -3' miRNA: 3'- -GCUUaagCGGGUaGCCUAG-------------UUGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 58362 | 0.66 | 0.983485 |
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Target: 5'- uGAcgUCGCUCGUCu--UCggUGAGGc -3' miRNA: 3'- gCUuaAGCGGGUAGccuAGuuGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 29326 | 0.66 | 0.985416 |
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Target: 5'- ------gGCCCcUCGGAUUu-CGAGGg -3' miRNA: 3'- gcuuaagCGGGuAGCCUAGuuGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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