Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18329 | 5' | -50 | NC_004681.1 | + | 44471 | 0.71 | 0.86522 |
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Target: 5'- cCGGAcgggCGCCCGUaGGGUCAGgucuCGGGGa -3' miRNA: 3'- -GCUUaa--GCGGGUAgCCUAGUU----GCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 50260 | 0.72 | 0.801391 |
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Target: 5'- gGAcgUCGCUCAUuugccgacuuggaCGGAgaugccgccgucgUCAGCGAGGg -3' miRNA: 3'- gCUuaAGCGGGUA-------------GCCU-------------AGUUGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 58723 | 0.72 | 0.78386 |
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Target: 5'- gCGAAUUCGaCCCGggCGaGAUCGuUGAGGu -3' miRNA: 3'- -GCUUAAGC-GGGUa-GC-CUAGUuGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 65998 | 0.74 | 0.689895 |
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Target: 5'- gCGG--UCGCCCA--GGAUCAugcGCGAGGa -3' miRNA: 3'- -GCUuaAGCGGGUagCCUAGU---UGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 48713 | 0.74 | 0.689895 |
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Target: 5'- uCGAG-UCGCCCG-CGG-UCAACGAGc -3' miRNA: 3'- -GCUUaAGCGGGUaGCCuAGUUGCUCc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 9471 | 0.8 | 0.407163 |
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Target: 5'- aGGAUggUGCCCAgcgGGAUCAGCGGGGu -3' miRNA: 3'- gCUUAa-GCGGGUag-CCUAGUUGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 58807 | 1.12 | 0.004015 |
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Target: 5'- uCGAAUUCGCCCAUCGGAUCAACGAGGa -3' miRNA: 3'- -GCUUAAGCGGGUAGCCUAGUUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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