Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18329 | 5' | -50 | NC_004681.1 | + | 18041 | 0.68 | 0.93817 |
Target: 5'- cCGGAgcCGCCC-UCGGcacUCAccuGCGGGGa -3' miRNA: 3'- -GCUUaaGCGGGuAGCCu--AGU---UGCUCC- -5' |
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18329 | 5' | -50 | NC_004681.1 | + | 15855 | 0.66 | 0.976526 |
Target: 5'- aGGucUCGCCCAUCuGGAUgccCAAgGAGu -3' miRNA: 3'- gCUuaAGCGGGUAG-CCUA---GUUgCUCc -5' |
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18329 | 5' | -50 | NC_004681.1 | + | 9471 | 0.8 | 0.407163 |
Target: 5'- aGGAUggUGCCCAgcgGGAUCAGCGGGGu -3' miRNA: 3'- gCUUAa-GCGGGUag-CCUAGUUGCUCC- -5' |
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18329 | 5' | -50 | NC_004681.1 | + | 8762 | 0.67 | 0.973787 |
Target: 5'- aGAGUUCGCg---CGGGUCuACGAGc -3' miRNA: 3'- gCUUAAGCGgguaGCCUAGuUGCUCc -5' |
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18329 | 5' | -50 | NC_004681.1 | + | 8590 | 0.66 | 0.976526 |
Target: 5'- gCGAAggCGCCCAUCaGcUCAGCcgcagccgugccGGGGu -3' miRNA: 3'- -GCUUaaGCGGGUAGcCuAGUUG------------CUCC- -5' |
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18329 | 5' | -50 | NC_004681.1 | + | 6985 | 0.66 | 0.976526 |
Target: 5'- aCGAAgcagGCCCAggauguUCGGAcccUCGuggACGAGGg -3' miRNA: 3'- -GCUUaag-CGGGU------AGCCU---AGU---UGCUCC- -5' |
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18329 | 5' | -50 | NC_004681.1 | + | 1341 | 0.66 | 0.976526 |
Target: 5'- aCGAGUUC-CCCAUCgguggGGAUCGGCu--- -3' miRNA: 3'- -GCUUAAGcGGGUAG-----CCUAGUUGcucc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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