Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18329 | 5' | -50 | NC_004681.1 | + | 58723 | 0.72 | 0.78386 |
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Target: 5'- gCGAAUUCGaCCCGggCGaGAUCGuUGAGGu -3' miRNA: 3'- -GCUUAAGC-GGGUa-GC-CUAGUuGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 23844 | 0.68 | 0.956564 |
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Target: 5'- -aGAUUCaGCCCAgcuGGAUCAGCGGc- -3' miRNA: 3'- gcUUAAG-CGGGUag-CCUAGUUGCUcc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 58445 | 0.67 | 0.964188 |
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Target: 5'- aGAccggCGCCCAcgucaagUGGAUCAACGAa- -3' miRNA: 3'- gCUuaa-GCGGGUa------GCCUAGUUGCUcc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 8762 | 0.67 | 0.973787 |
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Target: 5'- aGAGUUCGCg---CGGGUCuACGAGc -3' miRNA: 3'- gCUUAAGCGgguaGCCUAGuUGCUCc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 6985 | 0.66 | 0.976526 |
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Target: 5'- aCGAAgcagGCCCAggauguUCGGAcccUCGuggACGAGGg -3' miRNA: 3'- -GCUUaag-CGGGU------AGCCU---AGU---UGCUCC- -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 75161 | 0.66 | 0.976526 |
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Target: 5'- cCGGuaUCGCCUAcCGGGUCGAgGAc- -3' miRNA: 3'- -GCUuaAGCGGGUaGCCUAGUUgCUcc -5' |
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| 18329 | 5' | -50 | NC_004681.1 | + | 48713 | 0.74 | 0.689895 |
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Target: 5'- uCGAG-UCGCCCG-CGG-UCAACGAGc -3' miRNA: 3'- -GCUUaAGCGGGUaGCCuAGUUGCUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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