Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18330 | 3' | -58.7 | NC_004681.1 | + | 48671 | 0.66 | 0.539427 |
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Target: 5'- -cGGGaACCCugGCGGGCca-GCUCCCAu -3' miRNA: 3'- aaCUCcUGGG--CGUCCGuaaCGAGGGU- -5' |
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| 18330 | 3' | -58.7 | NC_004681.1 | + | 10030 | 0.67 | 0.518673 |
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Target: 5'- -cGuGGACCCGUacugcGGGuCAUcGCUCCUg -3' miRNA: 3'- aaCuCCUGGGCG-----UCC-GUAaCGAGGGu -5' |
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| 18330 | 3' | -58.7 | NC_004681.1 | + | 4048 | 0.67 | 0.498243 |
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Target: 5'- -gGAGGACCCGUggaacgAGGag-UGCUggcCCCAc -3' miRNA: 3'- aaCUCCUGGGCG------UCCguaACGA---GGGU- -5' |
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| 18330 | 3' | -58.7 | NC_004681.1 | + | 36086 | 0.67 | 0.488163 |
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Target: 5'- -cGcGGACCauguGCGGGaacgUGCUCCCAu -3' miRNA: 3'- aaCuCCUGGg---CGUCCgua-ACGAGGGU- -5' |
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| 18330 | 3' | -58.7 | NC_004681.1 | + | 57703 | 0.67 | 0.488163 |
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Target: 5'- -gGAGGGaCUGUAGGCGgcggacgGUUCCCAg -3' miRNA: 3'- aaCUCCUgGGCGUCCGUaa-----CGAGGGU- -5' |
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| 18330 | 3' | -58.7 | NC_004681.1 | + | 65033 | 0.69 | 0.384555 |
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Target: 5'- --aAGGACgCCGUAGGCAcUGCcaaggUCCCGu -3' miRNA: 3'- aacUCCUG-GGCGUCCGUaACG-----AGGGU- -5' |
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| 18330 | 3' | -58.7 | NC_004681.1 | + | 60092 | 1.05 | 0.000952 |
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Target: 5'- cUUGAGGACCCGCAGGCAUUGCUCCCAc -3' miRNA: 3'- -AACUCCUGGGCGUCCGUAACGAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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